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2 edition of Adenosine triphosphatase activity in gill tissue of rainbow trout (Salmo gairdneri Richardson) adapted to fresh water and salt water. found in the catalog.

Adenosine triphosphatase activity in gill tissue of rainbow trout (Salmo gairdneri Richardson) adapted to fresh water and salt water.

Edward Joseph Pfeiler

Adenosine triphosphatase activity in gill tissue of rainbow trout (Salmo gairdneri Richardson) adapted to fresh water and salt water.

by Edward Joseph Pfeiler

  • 163 Want to read
  • 14 Currently reading

Published .
Written in English

    Subjects:
  • Enzymatic analysis.

  • The Physical Object
    Paginationix, 95 l.
    Number of Pages95
    ID Numbers
    Open LibraryOL16768328M

    Whole body, gill, and liver copper uptake, gill Na+-K+-ATPase specific activity, and gill and liver acid-soluble thiols (AST), glutathione, and cysteine of rainbow trout (Salmo gairdneri) were   INTRODUCTION. The gill is the major iono-regulatory organ in teleosts. In the current model for active Na + uptake across tight epithelia, such as frog skin and the gills of freshwater (FW) teleosts, the driving force is thought to be set up by two membrane ion-pumps acting in series: an apical H +-ATPase and a basolateral Na +,K +-ATPase (Ehrenfeld and Garcia-Romeu, ; Avella and

      Gill Na+/K+-ATPase (NKA) in teleost fishes is involved in ion regulation in both freshwater and seawater. We have developed and validated rabbit polyclonal antibodies specific to the NKA α1a and α1b protein isoforms of Atlantic salmon (Salmo salar Linnaeus), and used western blots and immunohistochemistry to characterize their size, abundance and ://   THE EFFECTS OF LOW PH ON SALMONID EMBRYOGENESIS AND VITELLOGENESIS David B. Parker measuring gill ca2+-~~pase and in vitro gill osmotic water uptake from rainbow trout exposed in vivo. freshwater on gill Ca2+-Adenosine triphosphatase (ATP~S~) activity and osmotic water inflow in rainbow

      adenosine triphosphatase activity in the liver, muscle and brain of cichlid fish Sarotherodo«mossambicus (Peters) is studied. Results indicate a general decrease in the activity of aU the three pattern of decrease shows a direct linear relationship with salinity   Chronic Lead Exposure Protocol. The studies of chronic lead exposure were carried out using four 12 gal plastic aquaria (Eclipse R, Marineland, Moorpark, CA), with one control, lead-free aquarium (aquarium A) and three experimental aquaria in which concentrations of lead nitrate were adjusted to different dose were selected to elucidate the effect of a one order of


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Adenosine triphosphatase activity in gill tissue of rainbow trout (Salmo gairdneri Richardson) adapted to fresh water and salt water by Edward Joseph Pfeiler Download PDF EPUB FB2

A study of the thermal responses of Na-ATPase and NaK-ATPase activities in microsomes prepared from gill tissue of rainbow trout (Salmo gairdneri) revealed further evidence that the two activities are distinct from one another.

Arrhenius plots of the NaK-ATPase from sea water-adapted fish and the Na-ATPase from fresh water-adapted fish were linear (Fig. 4) with estimated activation Gill ATPase activities in the smallmouth bass it was found that "Na+-activation" represented a distinct activity and was not the result of a Na+-protected Mg-ATPase (unpublished results).

The difference in thermal stability between branchial Mg-ATPase in the trout and bass is interesting, especially since both species were adapted to the same 1.

The effects of zinc ions (Zn 2+) on the activities of Mg 2+- Ca 2+- Na +- K +- and Na + NH 4 +-ATPase in preparations of branchial microsomes from the gills of freshwater acclimated rainbow trout were studied.

Inhibition of all ATPases occurred from 1 to mg1 −1 Zn 2+ for assays conducted at 11°C. Na + NH 4 +-ATPase was the most sensitive to inhibition by Zn 2 Abstract. Gill tissue from eels adapted to fresh water or to sea water was disrupted in m-sucrose containing % (w/v) sodium deoxycholate and the subcellular distribution of (Na + +K +)-dependent adenosine triphosphatase was determined About 70% of the recovered enzyme was in a fraction sedimenting between g av.-min and g av.-min; the specific activities of enzymes   The following characteristics of the adenosine triphosphatases (ATPases) in the saccus vasculosus were studied in Salmo gairdneri Richardson: 1) distributional pattern, 2) cytochemical properties in relation to different substrates, inhibitors, pH and bivalent metal ions, and 3) ultrastructural localization.

Ultracytochemical studies using modifications of the Wachstein-Meisel technique showed Response of chloride cell numbers and gill Na'/K+ ATPase activity of freshwater rainbow trout (Salmo gairdneri Richardson) to salt feeding.

Aquaculture, Dietary NaCI up to the level of 12% was fed to freshwater rainbow trout ( g). The experiment was conducted between January and June The comparative activity of gill ATPase was examined in the bluegill sunfish (Lepomis macrochirus), fathead minnow (Pimephales promelas), and golden shiner (Notemigonus crysoleucas).Basal Na K ATPase activity was highest in bluegill sunfish ( μmol P i /mg protein/hr) and lowest in golden shiners ( μmol P i /mg protein/hr).

While a stimulation of Na K ATPase activity was observed at   Increases in branchial Na+/K+ ATPase activity during seawater adaptation of euryhaline fish species, have been well documented.

During the parr-smolt transformation of salmonids this activity increases two to five fold and is used as an indicator of the transformation.

In order to improve the understanding of differences in enzyme activity found between Atlantic salmonSalmo salar parr and 1. Freshwater nonanadromous rainbow trout, Salmo gairdneri, were injected three times a week with either saline, 10μg cortisol/g, μg thyroxine/g or 10μg cortisol/g + μg thyroxine/g during a period of 28 days (12 injections).A separate group was derived as a subgroup from the thyroxine group on day 14 and received Cortisol + thyroxine from day 14 until day 28 (six injections).

Watson TA, Beamish FW. The effects of zinc on branchial adenosine triphosphatase enzymes in vitro from rainbow trout, Salmo Gairdneri. Comp Biochem Physiol C. ; 68C (2)– Christensen GM, Tucker JH. Effects of selected water toxicants on the in vitro activity of fish carbonic anhydrase.

Chem Biol Interact. May; 13 (2)– Gill and kidney Mg2+-dependent, Na+:K+- and HCO3−-stimulated ATPase (EC ) activities were estimated at 25 °C and at acclimation temperature in rainbow trout, Salmo gairdneri, acclimated Elevated levels of Na + /K + adenosine triphosphatase α1A mRNA subunit expression was observed in the gill of rainbow trout acclimated to hypersaline conditions relative to freshwater animals.

No significant difference was noted in Na + /K + adenosine triphosphatase subunit levels in brains of either strain in freshwater or hypersaline ://   Sodium-potassium dependent adenosine triphosphatase activity in gills and kidneys of atlantic salmon (Salmo salar) Comparative Biochemistry and Physiology Part A: Physiology, Vol.

53, No. 4 Effects of hydrostatic pressure on gill Na-K-ATPase in an abyssal and a surfacing-dwelling teleost Sodium uptake by isolated-perfused gills of rainbow trout (Salmo gairdneri).

Comp Biochem Physiol. Mar 15; 33 (2)– Sargent JR, Thomson AJ, Bornancin M. Activities and localization of succinic dehydrogenase and Na-+/K-+-activated adenosine triphosphatase in the gills of fresh water and sea water eels (Anguilla anguilla). Influx rates of Na + from the water (A), gill sodium‐ and potassium‐activated adenosine triphosphatase activity (B), and gill silver levels (C) of control rainbow trout (hatched columns in A and Con in B and C) and those exposed to μg Ag/L (as AgNO 3) at different water sodium ://   Byline:and Yusuf Guner ABSTRACT In this study, rainbow trout (Oncorhynchus mykiss) ( +- g) and brown trout (Salmo trutta forma fario) ( +- g) were transferred to full-strength seawater ( g.l-1) for directly and gradually (21 days), then changes in gill Na+-K+-ATPase activity and size of chloride cells associated with environmental salinity were +OF+SEAWATER.

NEM-sensitive ATPase activity in the gill tissue of rainbow trout acclimated to various Na + and Ca 2+ levels (in mmol l 2 1) in the external medium for 10–14 days.

Mean ± :// Gill and liver microsomal Na+/K+-adenosine triphosphatase (ATPase) activities and plasma levels of 3,5,3'-triiodo-L-thyronine (T3) were measured in rainbow trout ( g) immersed in a   the gill epithelium of freshwater fish is a dynamic ion transporting epithelium that can regulate blood pH by manipulating the relative rates of Cl − and Na + uptake (8, 9, 11, 19,24).Current models suggest that Cl − uptake is linked to base extrusion, whereas transepithelial Na + transport is likely coupled to H + extrusion through an apical Na + channel electrogenically coupled to a V   adenosine triphosphatase activity (Glynn and Chir, ).

It has been shown that a (Na++K+) - dependent ATPase is found in almost all cell membranes, and its function is to transport Na+ out of the cell and K+ into the cell (Kinsolving,et al., ?article=&context=masters-theses.

Abstract. The activity of the enzyme Na +,K +-ATPase and morphological changes of gill chloride cells in grouper, Epinephelus coioides larvae and juveniles were determined 6–48 h after abrupt transfer from ambient rearing conditions (30–32 ppt, –30 °C) to different salinity (8, 18, 32, 40 ppt) and temperature (25, 30 °C) combinations.

Na +,K +-ATPase activity in day 20 larvae did The impact on the parameters of energy metabolism (the content of glucose (Glu) and lactate (LA), and the activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase(LDH)) in the muscle tissue, as well as on the ATP enzymatic system (ATP content, Total-ATPase, Na +-K +-ATPase, and Ca 2+-Mg 2+-ATPase) in the gill tissue of ?type=article&id=69_CJAS.

Different protein patterns in gill epithelium of a euryhaline and eurythermal teleost fish (Gillichthys mirabilis, Family Gobiidae) in response to long-term (2 months) osmotic and thermal acclimation were found for the first time.

Gill epithelial cells were isolated to remove extracellular proteins and quantify specialized cell types. Chloride cells were identified on the basis of size (>10